Listed are our core services. These are all performed at iPS Core. Price list can be found at the bottom of the page. We offer a wide range of additional services, these are in part performed at iPS Core, for information se "additional Services".
Package 1) Establishment of fibroblast cells from skin biopsies
This service takes about 1 month to complete and includes establishing fibroblast cells from a 2-4 mm skin biopsy in one T75 flask or three frozen vials.
Only freshly collected biopsies will be accepted. In the unlikely event that we fail to isolate fibroblasts from properly collected skin biopsies due to errors by the facility, there will be no charge for this attempt. We will ask the investigator to provide a new biopsy to complete the service.
Sendai mediated reprogramming
Package 2) Reprogramming human fibroblast cells using Sendai virus
This service takes about 3-4 months and includes:
- Mycoplasma testing, expansion, cryopreservation and set-up of fibroblast cells for reprogramming.
- Reprogramming using Sendai virus
- Re-plating of transduced cells onto one 6cm plate with human recombinant laminin coating, daily feeding.
- Manually picking of minimum 16 good looking colonies one months after transduction
- Expansion of colonies, feeding daily.
Two clones will be established and frozen. Minimum two vials of each established clone.
Basic iPS clone characterisation
Two iPS clones from each line, go through basic testing, that includes;
Test for expression of pluripotency factors through RT-PCR and immunocytochemistry. Test for loss of Sendai vector by RT-PCR. All data is delivered to customer. This is part of package 2.
Banking if iPS clones
Safe storage of your iPS clones in our nitrogen tank, .
Human iPS cell culturing courses
Each course is tailor-made for each customer, in terms of duration, content and dates. The courses are hands on iPS cells, together with our experienced lab technicians and the basic culturing technics such as thawing iPS cells, passaging and freezing iPS cells under feeder-free condition.
Price list for academic affiliations
|Service Package||short description||Price for Academic affliction (SEK)|
|1) Fibroblast derivation||Establishment of fibroblasts from skin biopsis||6 000|
|2) Reprogramming||Sendai virus mediated reprogramming and basic characterisation of two iPS clones.||41 500|
|3) Banking||Storage of frozen vials fibroblasts or iPS, price per vial and year||2 200|
|4) Training||Price per hour||250|
|5) Expansion of iPS Clone||Thawing, expansion and freezing of iPS clone, minimum 3 new vials||3 000|
Note on the Price list
The listed prices are excluded INDI. INDI of 22,3% will be added to all prices.
The price list is valid until December 31, 2015.
If you are a non-academic customer, please contact us for quotation.